S Chandy Pa, G Sridharana
a. Department of Clinical Virology, Christian Medical College, Vellore – 632 004, India.
The emerging viral diseases haemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS) are a cause of global concern as they are increasingly reported from newer regions of the world. The hantavirus species causing HFRS include Hantaan virus, Seoul virus, Puumala virus, and Dobrava-Belgrade virus while Sin Nombre virus was responsible for the 1993 outbreak of HCPS in the Four Corners Region of the US. Humans are accidental hosts and get infected by aerosols generated from contaminated urine, feces and saliva of infected rodents. Rodents are the natural hosts of these viruses and develop persistent infection. Human to human infections are rare and the evolution of the virus depends largely on that of the rodent host. The first hantavirus isolate to be cultured, Thottapalayam virus, is the only indigenous isolate from India, isolated from an insectivore in 1964 in Vellore, South India. Research on hantaviruses in India has been slow but steady since 2005. Serological investigation of patients with pyrexic illness revealed presence of anti-hantavirus IgM antibodies in 14.7% of them. The sero positivity of hantavirus infections in the general population is about 4% and people who live and work in close proximity with rodents have a greater risk of acquiring hantavirus infections. Molecular and serological evidence of hantavirus infections in rodents and man has also been documented in this country. The present review on hantaviruses is to increase awareness of these emerging pathogens and the threats they pose to the public health system.
Hantaviruses are described as emerging pathogens as newer serotypes are being discovered in many areas non endemic to Hantaviruses (Morse and Schluederberg 1990). Increased globalization and trade, evolution of viral pathogens to adapt to new hosts and increased habitat modification are a few factors responsible for their emergence (Ulrich et al 2002).
Hantaviruses are the most widely distributed zoonotic rodent-borne viruses (Johnson 2001) and can cause two important clinical syndromes: haemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) in Asia and the Americas respectively (Krüger et al 2001). HPS is also referred to as hantavirus cardiopulmonary syndrome (HCPS) as deaths have been attributed to cardiac failure rather than pulmonary edema (Tager et al 2003). Currently up to 21 species and more than 30 genotypes of hantaviruses have been described (van Regenmortel et al 2000). The important species include Hantaan virus (HTNV), Seoul virus (SEOV), Puumala virus (PUUV), Sin Nombre virus (SNV) and Dobrava-Belgrade virus (DOBV) (Lednicky 2003).
Structure and morphology of Hanta viruses
Hantaviruses are enveloped RNA viruses (family Bunyaviridae, genus Hantavirus) that have a negative-sense, tri-segmented genome (Schmaljohn and Dalrymple 1983).
The large (L) segment codes for the viral RNA-dependent RNA polymerase (RdRp) and the medium (M) segment for the glycoprotein precursor (GPC) which is processed into the two envelope glycoproteins (G1 and G2). The small (S) segment codes for the nucleocapsid (N) protein (Schmaljohn et al 1986; Schmaljohn 1990). The hantavirus particle is spherical with a diameter ranging from80-120 nm and consists of three circular nucleocapsids, each contains one RNA segment complexing with N and RdRp proteins (Lee and Cho1981; Severson et al 2005)
The 32 and 52 termini of all the three RNA segments are conserved and complementary thus forming pan- handle structures, this being an important feature of the family Bunyaviridae (Elliot et al 1991). The pan-handle structures are about 17 base pairs long and may regulate viral transcription and replication (Flick and Pettersson 2001; Blakqori et al 2003). The L segment is approximately 6500 nucleotides (nt) long with a single open reading frame (ORF) coding for about 2150 amino acids. The M segment is approximately 3700 nt long and codes for about1140 amino acids while the S segment is about1700 nt long coding for about 430 amino acids (Plyusnin et al 1996). In the S segment of hantavirus species carried by rodents of the sub- families, Arvicolinae and Sigmodontinae, there is an additional ORF coding for a non-structural protein, this is missing in serotypes hosted by the Murinae rodents. NS proteins have not been detected in hantavirus-infected cells (Parrington and Kang 1990; Stohwasser et al 1990; Spiropoulouet al 1994). The 32 non-coding region (NCR) of the hantavirus S segment varies in length from 300-700 nucleotides among the different serotypes (Plyusnin 2002).
Hantavirus N protein is the most abundant viral protein in the virion and infected cells where it is found in the cytoplasm, forming inclusion bodies and filamentous structures (Vapalahti et al 1995; Ravkov et al 1998). It plays a central role in replication and is expressed in large quantities in infected cells (Flick and Pettersson 2001; Blakqori et al 2003). The N protein protects the RNA genome by encapsidation. It is a multifunctional protein with additional functions other than viral RNA encapsidation, protection, replication and virus assembly. It also seems to interact with cellular macromolecules to inhibit important cellular regulatory pathways (Kaukinen et al 2005). The N protein is highly antigenic and antibodies are produced early in infection as there is a strong and rapid immune response (Elgh et al 1996). The N- terminal 100 amino acids of the N protein are highly antigenic and practically all sera positive for hantavirus antibodies recognize this region. This portion of the N protein is used in diagnostic assays to diagnose hantavirus infections. The middle part of this protein (210-310) is highly variable within different hantavirus species. The C terminus is also highly conserved and is known to bind RNA. B-cell epitopes are seen in the N terminal region while T- cell epitopes are distributed randomly (Jenison et al1994; Lundkvist et al 1995; Vapalahti et al 1995; Elgh et al 1996). Within a given hantavirus serotype, the primary structure of the N protein is generally conserved (Kaukinen et al 2005). Recombinant N protein is currently being commercially expressed in Escherichia coli, baculovirus, insect and yeast systems for use in diagnostic assays (Schmaljohn et al 1990).
The GPC (1132-1148aa) is not found in infected cells indicating that it is cleaved into Gn and Gc proteins (formerly called G1 and G2). GPC is synthesized on the endoplasmic reticulum membrane where it is cleaved, glycosylated and forms heterodimers which are transported to the Golgi complex (Schmaljohn et al 1987; Löber et al 2001). Accumulation of Gn and Gc proteins on the Golgi complex provides the budding site for hantaviruses (Schmaljohn et al 1986). These glycoproteins mediate cell attachment and fusion (Arikawa et al 1985). The L protein is a RNA transcriptase and replicase seen in viruses that have to transcribe mRNA and replicate genomic RNA from antigenomic RNA. So it should have endonuclease, transcriptase and replicase activities. The L protein has a single ORF. The L and N proteins are required for RNA synthesis (Kukkonen et al 2005).
Diversity of Hantaviruses
The N protein between Hantavirus serotypes shows greater degree of cross reactivity than the G1 and G2 proteins (Jenison et al 1994). There is a high degree of antigenic cross reactivity between members belonging to the three groups of hantaviruses namely:
- HTNV–like viruses (HTNV, SEOV, DOBV, Thailand virus [THAIV]) carried by Murinae rodents (Old world rats and mice).
- PUUV-like viruses (PUUV, Tula virus [TULV]) carried by Arvicolinae rodents (voles and lemmings).
- SNV-like viruses (SNV, Andes virus [ANDV]) carried by Sigmodontinae rodents (Vapalahti et al 2001).
There is a great degree of cross reactivity between SNV and PUUV group of viruses. The amino acid sequences of the N protein terminal regions show 83.7% to 90.7% homology among the HTNV-like viruses and 74.4% homology for SNV and PUUV. The overall amino acid homologies between the HTNV-like viruses and the other two groups are from 44.2% to 46.5% (Elgh et al 1997). This antigenic cross reactivity was thought to be sufficient for assays with two antigens of HTNV and PUUV and one SNV antigen for serological diagnosis of Hantavirus infections in Eurasia and America respectively. However, later studies have shown that 7.1% of DOBV positive acute phase samples and 12.5% of DOBV positive convalescent samples were negative with heterologous antigen based assays. This emphasizes the usefulness of homologous antigens in serological assays (Kallio-Kokko et al 2000).
The phylogenetic analysis of the N protein also groups Hantaviruses in the same way as given above. The Phylogenetic trees show two major lineages of hantaviruses, one branching to HTNV, SEOV, THAIV and DOBV and the other branch leading to PUUV, SNV and other New World hantaviruses. Thottapalayam virus (TPMV), the most divergent member of this genus shows greater genetic relatedness to HTNV- like virus group (A Toney, B Meyer and C Schmaljohn, unpublished data). Phylogenetic trees using the L and M segment sequences also show identical branching suggesting that similar evolutionary events have occurred for all genome segments (Xiao et al 1994; Plyusnin et al 1996).
The plaque-reduction neutralization method is the most reliable assay for serotyping (Chu et al 1994). The M segment gene products (surface proteins) are more prone to mutations and its analysis at the nucleotide and amino acid level would be a more sensitive approach to classification of Hantaviruses (Xiao et al 1994). Since humans are accidental hosts of Hantaviruses, human epidemics of Hantavirus disease do not contribute to the virus evolution (Avsic- Zupanc et al 1995).
Of all the Hantaviruses known, SEOV is genetically the most homogenous. Regardless of the geographical origin, the deduced M segment amino acid sequences of SEOV isolates show99% homology (Xiao et al 1994). HTNV is known to be stable and isolates are known to show M segment amino acid homology of 97% (Schmaljohn et al 1988). PUUV isolates are the most variable and amino acid homology of the M segment may range from 83%-94% (Jay et al1997). Persistent infections of the rodent host provide opportunity for two mechanisms responsible for virus evolution: genetic drift (Spiropoulou et al 1994) and genetic shift (Henderson et al 1995).
Old World and New World Hantaviruses
Hantavirus are classified into two main groups: Old World and New World hantaviruses. The Old World hantaviruses include species which cause HFRS in Asia and Europe while the New World hantaviruses cause HCPS in the Americas (Mertz et al 2006; Muranyi et al 2005). The first New World hantavirus was identified during the outbreak of HCPS in the Four Corners Region of southwestern USA in 1993. The etiological agent was SNV; since then many pathogenic New World hantavirus species have been identified and characterized (Khaiboullina et al 2005). The New World hantaviruses are associated with rodents belonging to the Sigmodontinae subfamily (Montgomery et al 2007) while the Old World hantaviruses are associated with rodents of the subfamilies Murinae and Arvicolinae (Peters and Khan 2002). The distribution and known rodent hosts of Hantaviruses are shown in table 1.
Clinical features of Hantaviruses
The clinical picture and severity of HFRS and HCPS depends on the infecting species.
The incubation period is 1-5 weeks and the onset of the disease is with fever and influenza-like symptoms (Schmaljohn and Hjelle1997; Muranyi et al 2005). Hemorrhagic manifestations if present are seen as flushing of the face, injection of the conjunctiva and mucous membranes (Kruger et al 2001). The disease is conveniently described as having five phases: a febrile phase lasting 3-5 days followed by a hypotensive (shock) phase lasting from a few hours to a few days, a subsequent oliguric phase lasting 3-7 days and finally a diuretic phase leading to the convalescent phase (McCaughey and Hart 2000).
The febrile phase is characterized by development of an acute influenza-like illness. Nausea, vomiting, back and abdominal pain are additional findings. Proteinuria suddenly appears on the third to fifth day. In severe HFRS which is often seen in endemic areas, on the 4th day there may be onset of albuminuria. Towards the end of the febrile phase flushing of the face and conjunctival suffusion may be seen. These symptoms may mark the onset of the hypotensive phase characterized by thrombocytopenia. One-third of deaths occurring during this phase are due to shock. Proteinuria may persist. Almost half the fatalities occur during the subsequent oliguric phase due to renal failure. Survivors progress to the diuretic phase which may last from few days to few weeks and is associated with improvement of renal functions: then to a final convalescent phase during which the patient recovers completely (Peters et al 1999; Krüger et al 2001; Lednicky 2003). During evaluation of patients with suspected Hantavirus infections, a physician may encounter mainly three kinds of clinical presentations; fever with shock and multi-organ failure, fever with oliguric acute renal failure and febrile illness without any renal failure (Bruno et al 1990).
In patients with severe HFRS the blood picture shows haemoconcentration, leucocytosis and thrombocytopenia. Urine analysis shows proteinuria, hematuria and pyuria. Significant elevations of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and creatine kinase enzymes are frequently noticed. Marked polymorphonuclear leukocytosis with a left shift and CD8+ activated cells appear as atypical lymphocytes on a peripheral blood smear (Peters et al, 1999). In patients with acute renal failure: fever, abdominal pain, azotemia and thrombocytopenia were common clinical findings. Urinanalysis may show proteinuria and hematuria. Elevations in serum aspartate and alanine aminotransferase are moderate and common. Oliguric renal failure is common in most of the patients. In patients presenting with undifferentiated febrile illness, thrombocytopenia is commonly seen; most have increased serum aspartate and alanine aminotransferase values. Urine analysis of some may show proteinuria, hematuria and pyuria. Recovery is complete and uncomplicated. These cases can be mistaken for influenza, viral hepatitis or streptococcal pharyngitis (Bruno et al 1990; Mertz 2002).
The clinical picture and severity of HFRS may vary for different hantavirus species. HFRS caused by HTNV and DOBV may show a 5%-15% mortality; SEOV infections are milder with greater liver involvement (Kim et al 1995). PUUV causes nephropathia epidemica (NE), the mildest form of HFRS (Nichol 2001).
Fever and thrombocytopenia are common clinical findings and important clues to diagnosis of HFRS. Other signs that may help in the initial diagnosis of HFRS would be conjunctival hemorrhage, facial flushing and petechiae. Jaundice is noticeably absent and helps to differentiate hantavirus infections from leptospirosis, viral hepatitis or bacterial sepsis. However there has been a report of hantavirus infection in patients presenting with febrile illness and jaundice (Gloriani- Bargaza et al 1999). Maculopapular rash is absent and helps differentiate between hantavirus infection, leptospirosis, dengue fever or scrub typhus (Markotic et al 2001). It has been reported that Apodemus agrarius, Cricetulus triton and Rattus norvegicus are the common rodent hosts of both scrub typhus and HFRS in Northern China. Therefore the possibility exists for dual infections of HFRS and scrub typhus (Houck et al 2001; Heung-Chul et al 2007; Liu et al 2007).
HCPS can be described to occur in two phases, prodromal and cardiopulmonary phase. The prodromal stage is characterized by fever, headache, chills and myalgia. Like HFRS the clinical picture at this phase may be confused with other viral infections.
The onset of the cardiopulmonary phase is with pulmonary edema, dyspnea and hypoxemia. Patients with severe illness may progress to cardiac depression, respiratory failure and acidosis leading to fatal arrhythmias (Nichol2001). Common laboratory indicators of HCPS include thrombocytopenia and hemoconcentration; presence of atypical lymphocytes is also a common finding (Peters and Khan 2002). Different hantaviruses can cause HCPS with different clinical presentations. Renal involvement in SNV infection is rare but renal insufficiency has been reported in infections with ANDV, Bayou virus (BAYV) and Black Creek Canal virus (BCCV) (Duchin et al 1994; Hjelle et al 1996; Khan et al1996). Table 2 highlights differentiating features between HFRS and HCPS.
HFRS and HCPS are uncommon in children. However there have been occasional reports of NE and HCPS in children. The clinical picture of pediatric HCPS cases closely mimics that of adults with fatality rate close to 33% (Overturf 2005). The diagnosis of hantavirus infections in newer geographical areas can be improved if clinicians are aware of the protean manifestations of hantavirus disease.
Laboratory diagnosis of hantavirus infections
It is difficult to diagnose hantavirus infections clinically as the early signs and symptoms of the disease are non-specific (Nichol 2001). Serology is the mainstay of diagnosis of hantavirus infections as the viremia in HFRS patients is short-lived (Papa et al 1998). Since there is considerable cross-reactivity between related hantaviruses, antigens used for serological assays help in identifying hantaviruses but not always the infecting serotype (Wichman et al 2001). However a combination of clinical and serological findings is generally used (Muranyi et al 2005). There is an early and strong humoral response against the N antigen, hence is the antigen of choice for enzyme linked immunoassays (ELISA) (Zoller et al1993). The use of native N antigen has given way to recombinant entire and truncated N antigens produced in prokaryotic and eukaryotic vector systems (Sjolander et al 1997). However homologous antigens are far more sensitive than heterologous antigens and are advisable in areas where circulating serotypes are known (Sjolander and Lundkvist1999). Immunofluorescence assays (IFA) are also commonly used but have lower specificity. These problems with IFA make them generally unsuitable for serological surveys (Groen et al 1991).
Detection of virus-specific IgM is advocated for diagnosis of acute hantavirus infections. Preferably the ì- capture ELISA should be used as it has a higher specificity than IFA and indirect antibody detection ELISA (Krüger et al 2001). In non-endemic areas presence of virus specific IgM and IgG could be useful for diagnosis; however IgG response may sometimes be delayed (Lundkvist et al 1995, 1997). Positive results on single serum samples should be correlated with clinical information (Wichman et al 2001). Nested reverse transcriptase- polymerase chain reaction (RT- PCR) using genus and species-specific primers are employed for diagnosis of hantavirus infections (McCaughey and Hart 2000). Hantaviruses are routinely cultured in Vero E6 cell line (African green monkey kidney cell line) in which it does not readily cause any cytopathic effect. The virus is detected in cell culture by IFA. Isolation of hantaviruses from clinical specimens is difficult and hazardous as it should be performed in biosafety level-3 laboratories. In general rodent isolates are commoner than human isolates.
Epidemiology of hantavirus infections
The epidemiology and geographical distribution of hantavirus species closely reflects that of their rodent reservoirs (Khaiboullina et al 2005). Hantavirus infections of the rodent cause an asymptomatic and persistent infection. These viral agents may then spill over to other animal hosts like man resulting in serious disease but with clearance of virus in survivors (Nichol 2001). It is known that in humans, asymptomatic or non-specific mild infections may occur more frequently than symptomatic infections (McCaughey and Hart2000). However, since humans are not the natural host reservoir for hantaviruses and infection is accidental, man is a dead-end host for the virus (Muranyi et al 2005). Therefore the entire amplification and perpetuation of the virus relies on efficient transmission cycles between rodents.
Transmission of infection is via the aerosols generated from virus-contaminated rodent feces, urine or saliva (Lundkvist and Niklasson 1994). Hantavirus transmission among rodents occurs through bites and may also result in human infection (Glass et al 1988; Dournon et al 1984). Transmission of infection may also probably occur via food or hands contaminated by rodent excreta or via rodent bites or scratches. Human to human transmission of hantaviruses was considered unlikely until reports of an outbreak of ANDV from Argentina changed this perception (Wells et al 1997). This outbreak report from Argentina was also the sole example of a nosocomial transmission of hantaviruses.
There is an occupational risk of transmission of hantavirus infections with animal trappers, mammalogists, forest workers, farmers and military personnel at greater risk (Goren et al 1995, Schmaljohn and Hjelle 1997). Pediatric infections are uncommon and men are more often infected than women as an occupational risk (McCaughey and Hart 2000). Laboratory and animal facility- acquired hantavirus infections due to contact with infected animals and infected cell lines has been reported in laboratory personnel (Lee and Johnson1982; Lloyd et al 1984). HFRS and HPS are basically rural diseases (Young et al 1998; Mertz 2002); SEOV is cosmopolitan in nature and known to cause urban cases of HFRS (Kruger et al 2001). In Asia, the incidence of hantavirus infections increase during summer and spring and peak during fall because of greater contact between rodents and man during these planting and harvesting months. However in Sweden, the peak incidence of the disease occurs during the first frost in winter when rodents may take shelter in human dwellings (van Ypersele 1991).
In the Old World, the majority of hantavirus cases have been reported from Europe and Eastern Russia (China, Korea and Far East Russia). The prototype, HTNV is vectored by the mouse species Apodemus agrarius, a mouse found in the fields. About 10,000 cases of HTNV related HFRS cases are seen in China every year, while in Korea about300-900 cases occur annually. HTNV related HFRS cases occur during the fall when there is an increased farming activity and there is rodent movement into human dwellings to escape the cold. Most SEOV circulates in the domestic rats and causes urban cases of HFRS. It is worldwide in distribution with the distribution of the hosts through international shipping routes. The majority of SEOV- related HFRS occurs during spring and early summer. DOBV causes serious HFRS in the Balkans and in southern parts of Europe. The primary reservoir is Apodemus flavicollis. DOBV- associated HFRS occur in the late spring and summer months and in rural areas.
NE is a mild form of HFRS and is caused by PUUV. The virus is found throughout Scandinavia and in parts of Europe west of the Ural Mountains. The host of this species is the bank vole (subfamily Arvicolinae), Clethrionomys glareolus. The HCPS in the Four Corners Region of the US in 1993 and the subsequent discovery of the etiological agent, the SNV marked the beginning in the search for hantavirus species. SNV is vectored by the deer mouse, Peromyscus maniculatus which belongs to the subfamily Sigmodontinae. The 1993 outbreak of HCPS was as a result of increase of deer mice populations owing to the prolonged to the El Niòo effect. The increased rain promoted the increase of deer mice populations. The peak of HPS cases occur in late spring and summer and about equal number of males and females are infected. As in other hantavirus infections, pediatric cases are rare. Although initially regarded as new disease, retrospective studies show that HCPS cases have gone unnoticed since 1959 in USA. (Kruger et al 2001; Lednicky 2003).
Hantaviruses as agents of biological warfare
The outbreak of HCPS in the Four Corners Region in 1993 raised many rumors that the etiological agent was a deadly virus that had escaped from a military laboratory involved in bio-weapons research. Retrospective studies showed that the disease was around since 1959. In the list of pathogens listed as biowarfare agents, hantaviruses are included in list C which has the lowest potential for use as a bioterrorist weapon. In man, viremia is short-lived and hantavirus isolates from rodents are commoner that those from humans. HTNV viremia occurs during the prodromal stage and recovery of virus from human samples is poor. The host- related cellular immune response rather than the pathogenic capacity of the virus per se are responsible for development of the disease. The aerosol route is the only efficient way of transmission and the survival of the free virus in the environment is doubtful. Human to human transmission has been documented only in one case from Argentina. Some hantavirus infections are treatable and formalin- inactivated vaccines have been in use for more than a decade in Korea. The above mentioned features of hantavirus make it unsuitable for use as a biowarfare agent (Clement 2003).
Hantavirus infections in rodents
Unlike other members of its genus Bunyaviridae which are arboviruses, hantaviruses are maintained in nature in specific rodent hosts (roboviruses). The geographical distribution of hantavirus species closely reflect that of the reservoir rodent host and hantavirus species in a particular geographical region are closely related (Meyer and Schmaljohn 2000). The phylogenetic trees for hantavirus species and their hosts show amazing congruence thus proving that there has been co-evolution and co-speciation of hantavirus species and their hosts (Plyusnin et al 1996; Vapalahti et al2001). The natural reservoir of hantaviruses are murid rodents (order Rodentia; family Muridae; subfamilies Murinae, Arvicolinae and Sigmodontinae). We do not know yet whether these non- rodent hosts merely represent spill-over hosts which might not have much role in transmission of hantaviruses (Young et al 1998). Experiments in laboratory animals have shown that young susceptible rodents develop life long infections. In the adult animal, the infection is transient and may get cleared naturally (Nakamura et al 1985; Kim et al 1995).
The absence of an arthropod vector is an important reason for the rigid association of hantavirus species with specific rodent hosts which are persistently infected. It is the reason also for the slow geographical movement of hantavirus species as there is only a moderate efficiency in the natural horizontal rodent-rodent transmission of hantaviruses. It also limits movement of hantavirus to other host species.
Treatment and prevention
There are no effective anti-viral drugs for the treatment of all hantavirus infections. Ribavirin (1- â-D-ribofuranosy l-1, 2, 4-triazole-3-carboxamide) has been used in clinical trials for treatment of HFRS patients in the People’s Republic of China and has shown reduction in fatality. However, it remains ineffective for treatment of HPS. Supportive therapy is the best to control progression towards life threatening symptoms (Krüger et al 2001; Nichol 2001). Prevention of exposure to rodent excreta is the best way to avoid infection. Simple preventive steps are: decontamination of human dwelling having signs of rodent activity, maintaining rodents as pets should be discouraged and proper storage of food should be practiced. There are a few inactivated vaccines (Hantavax) licensed for use in Korea but the protective response is short lived. Baculovirus and vaccinia-expressed hantavirus glycoproteins confer protection in animal models. There is ongoing research on nucleic acid vaccines (Krüger et al 2001; Nichol 2001).
Hantaviruses research in India with special reference to the Thottapalayam virus
It was in 1966 that Thottapalayam virus (TPMV), the first indigenous hantavirus species was isolated from the spleen of a shrew (insectivore), Suncus murinus, captured in South India during field studies of Japanese encephalitis (Carey et al 1971). Initially believed to be an arbovirus, this isolate was later proved to be a member of the family Bunyaviridae and genus Hantavirus, based on electron microscopic and serological studies (Zeller et al 1989). However, TPMV is one of the few hantavirus isolates which has been isolated in a non- rodent host.
Chandy et al (2005) in a study of 152 serum samples found 23 (14.7%) individuals with febrile illness positive for anti-hantavirus IgM and5.7% of healthy blood donor samples tested were positive. These findings suggested the presence of hantavirus infections in the Indian population, presenting as symptomatic or asymptomatic infections.
Evidence exists for SEOV-like infection in12% and a PUUV-like infection in 5% of Indians presenting with a leptospirosis-like clinical picture from Cochin and Chennai area of India (Clement et al 2006). In 2007 there were reports from Western India of hantavirus infections presenting with ocular involvement (Mehta and Jiandani 2007).
TPMV is phylogenetically and antigenically quite distinct from the other hantaviruses and it has probably co-evolved with its non-rodent host (Yadav et al 2007). The pathogenicity of TPMV was doubtful as the host insectivore was believed to be a spillover host. Anti-TPMV antibodies in sera from shrews in Indonesia and in a patient presenting with acute febrile illness of unknown etiology may be proof of Suncus murinus being the natural host of TPMV and of causing human infections(Okumuraetal2007).
Sero-epidemiological studies have indicated a 4% seropositivity of hantavirus infections in India. It has been proved that tribes (Irulas) living in close proximity with rodents show significantly higher frequency of antibody positive status. Patients with chronic renal disease appear to have a higher rate of hantavirus seropositivity compared to the healthy blood donor control group. Warehouse workers (god own workers) showed a low seropositivity reflecting a lower risk of hantavirus infections (Chandy et al 2008).
Our own experience with studies on hantaviruses show that THAIV -like, HTNV–like/SEOV-like species are circulating in India (our unpublished data). Partial S gene segment sequences recovered from patients with acute febrile illness show 93%–97% homology with HTNV.
In our laboratory, 54 rodent sera were tested by IFA using HTNV antigen. Of the 54 tested, 9 were seropositive by IFA; 6 of the 9 were sero reactive by WB using THAIV antigens. The partial S segment from a seropositive animal was amplified from the lung tissue and it showed 93% identity to HTNV S segment (our unpublished data). Considerable research still needs to be done to characterize the circulating species, to extensively study rodent reservoirs in India and to develop of better diagnostic tools (serological and molecular) for rapid diagnosis of hantavirus infections which will further our knowledge of these emerging and reemerging viral agents.
- Dr. S Chandy P, Department of Clinical Virology, Christian Medical College, Vellore
- Dr. G Sridharan, Department of Clinical Virology, Christian Medical College, Vellore – 632 004, India.
Conflict of Interest: None declared
- Arikawa J, Takashima I, Hashimoto N. Cell fusion by haemorrhagic fever with renal syndrome (HFRS) viruses and its application for titration of virus infectivity and neutralizing antibody. Arch Virol. 1985; 86(3-4):303–13. [Pubmed]
- Avsic-Zupanc T, Toney A, Anderson K, Chu YK, Schmaljohn C. Genetic and antigenic properties of Dobrava virus: a unique member of the Hantavirus genus, family Bunyaviridae. J Gen Virol. 1995 Nov; 76 ( Pt 11):2801–8. [Pubmed] | [Crossref]
- Blakqori G, Kochs G, Haller O, Weber F. Functional L polymerase of La Crosse virus allows in vivo reconstitution of recombinant nucleocapsids. J Gen Virol. 2003 May; 84(Pt 5):1207–14. [Pubmed] | [Crossref]
- Bruno P, Hassel LH, Brown J, Tanner W and Lau A 1990. The protean manifestations of hemorrhagic fever with renal syndrome. A retrospective review of 26 cases from Korea; Ann. Internal Med. 113 385–391
- Carey DE, Reuben R, Panicker KN, Shope RE, Myers RM. Thottapalayam virus: a presumptive arbovirus isolated from a shrew in India. Indian J Med Res. 1971 Nov; 59(11):1758–60. [Pubmed]
- Chandy S, Mitra S, Sathish N, Vijayakumar TS, Abraham OC, Jesudason MV, et al. A pilot study for serological evidence of hantavirus infection in human population in south India. Indian J Med Res. 2005 Sep; 122(3):211–5. [Pubmed]
- Chandy S, Yoshimatsu K, Ulrich RG, Mertens M, Okumura M, Rajendran P, John GT, Balraj V, Muliyil J, Mammen J, Abraham P, Arikawa J and Sridharan G 2008Seroepidemiological study on hantavirus infections in India; Trans. R. Soc. Trop. Med. Hyg.102 70–74. [Pubmed] | [Crossref]
- Chu YK, Rossi C, Leduc JW, Lee HW, Schmaljohn CS, Dalrymple JM. Serological relationships among viruses in the Hantavirus genus, family Bunyaviridae. Virology. 1994 Jan; 198(1):196–204. [Pubmed] | [Crossref]
- Clement JP. Hantavirus. Antiviral Res. 2003 Jan; 57(1-2):121–7. [Pubmed] | [Crossref]
- Clement J, Maes P, Muthusethupathi M, Nainan G, van Ranst M. First evidence of fatal hantavirus nephropathy in India, mimicking leptospirosis. Nephrol Dial Transplant. 2006 Mar; 21(3):826–7. [Pubmed] | [Crossref]
- Douron E, Moriniere B, Matheron S, Girard PM, Gonzalez JP, Hirsch F, et al. HFRS after a wild rodent bite in the Haute-Savoie–and risk of exposure to Hantaan-like virus in a Paris laboratory. Lancet. 1984 Mar 24; 1(8378):676–7. [Pubmed]
- Duchin JS, Koster FT, Peters CJ, Simpson GL, Tempest B, Zaki SR, et al. Hantavirus pulmonary syndrome: a clinical description of 17 patients with a newly recognized disease. The Hantavirus Study Group. N Engl J Med. 1994 Apr 7; 330(14):949–55. [Pubmed] | [Crossref]
- Elgh F, Lundkvist A, Alexeyev OA, Stenlund H, Avsic-Zupanc T, Hjelle B, et al. Serological diagnosis of hantavirus infections by an enzyme-linked immunosorbent assay based on detection of immunoglobulin G and M responses to recombinant nucleocapsid proteins of five viral serotypes. J Clin Microbiol. 1997 May; 35(5):1122–30. [Pubmed] | [Source]
- Elgh F, Lundkvist A, Alexeyev OA, Wadell G, Juto P. A major antigenic domain for the human humoral response to Puumala virus nucleocapsid protein is located at the amino-terminus. J Virol Methods. 1996 May; 59(1-2):161–72. [Pubmed]
- Elliott RM, Schmaljohn CS, Collett MS. Bunyaviridae genome structure and gene expression. Curr Top Microbiol Immunol. 1991; 169:91–141. [Pubmed]
- Flick R, Pettersson RF. Reverse genetics system for Uukuniemi virus (Bunyaviridae): RNA polymerase I-catalyzed expression of chimeric viral RNAs. J Virol. 2001 Feb; 75(4):1643–55. [Pubmed] | [Crossref]
- Glass GE, Childs JE, Korch GW, LeDuc JW. Association of intraspecific wounding with hantaviral infection in wild rats (Rattus norvegicus). Epidemiol Infect. 1988 Oct; 101(2):459–72. [Pubmed]
- Gloriani –Bargaza N, Cabanban A, Graham R R and Florese R H 1999 Hantavirus infection in two Filipino patients with jaundice; Philos. J. Microbiol. Infect. Dis. 28 21–23. [Source]
- Groen J, Gerding MN, Jordans JG, Clement JP, Nieuwenhuijs JH, Osterhaus AD. Hantavirus infections in The Netherlands: epidemiology and disease. Epidemiol Infect. 1995 Apr; 114(2):373–83. [Pubmed]
- Groen J, Jordans HG, Clement JP, Rooijakkers EJ, UytdeHaag FG, Dalrymple J, et al. Identification of Hantavirus serotypes by testing of post-infection sera in immunofluorescence and enzyme-linked immunosorbent assays. J Med Virol. 1991 Jan; 33(1):26–32. [Pubmed]
- Henderson WW, Monroe MC, St Jeor SC, Thayer WP, Rowe JE, Peters CJ, et al. Naturally occurring Sin Nombre virus genetic reassortants. Virology. 1995 Dec 20; 214(2):602–10. [Pubmed] | [Crossref]
- Kim H-C, Klein TA, Chong S-T, Collier BW, Usa M, Yi SC, et al. Seroepidemiological survey of rodents collected at a U.S. military installation, Yongsan Garrison, Seoul, Republic of Korea. Mil Med. 2007 Jul; 172(7):759–64. [Pubmed]
- Hjelle B, Goade D, Torrez-Martinez N, Lang-Williams M, Kim J, Harris RL, et al. Hantavirus pulmonary syndrome, renal insufficiency, and myositis associated with infection by Bayou hantavirus. Clin Infect Dis. 1996 Sep; 23(3):495–500. [Pubmed] | [Source]
- Houck MA, Qin H, Roberts HR. Hantavirus transmission: potential role of ectoparasites. Vector Borne Zoonotic Dis. 2001; 1(1):75–9. [Pubmed]
- Jay M, Ascher MS, Chomel BB, Madon M, Sesline D, Enge BA, et al. Seroepidemiologic studies of hantavirus infection among wild rodents in California. Emerging Infect Dis. 1997 Jun; 3(2):183–90. [Pubmed] | [Crossref]
- Jenison S, Yamada T, Morris C, Anderson B, Torrez-Martinez N, Keller N, et al. Characterization of human antibody responses to four corners hantavirus infections among patients with hantavirus pulmonary syndrome. J Virol. 1994 May; 68(5):3000–6. [Pubmed]
- Johnson KM. Hantaviruses: history and overview. Curr Top Microbiol Immunol. 2001; 256:1–14. [Pubmed]
- Kallio-Kokko H, Lundkvist A, Plyusnin A, Avsic-Zupanc T, Vaheri A, Vapalahti O. Antigenic properties and diagnostic potential of recombinant dobrava virus nucleocapsid protein. J Med Virol. 2000 Jun; 61(2):266–74. [Pubmed] | [Crossref]
- Kaukinen P, Vaheri A, Plyusnin A. Hantavirus nucleocapsid protein: a multifunctional molecule with both housekeeping and ambassadorial duties. Arch Virol. 2005 Sep; 150(9):1693–713. [Pubmed] | [Crossref]
- Khaiboullina SF, Morzunov SP, St Jeor SC. Hantaviruses: molecular biology, evolution and pathogenesis. Curr Mol Med. 2005 Dec; 5(8):773–90. [Pubmed] | [Source]
- Khan AS, Gaviria M, Rollin PE, Hlady WG, Ksiazek TG, Armstrong LR, et al. Hantavirus pulmonary syndrome in Florida: association with the newly identified Black Creek Canal virus. Am J Med. 1996 Jan; 100(1):46–8. [Pubmed]
- Kim YS, Ahn C, Han JS, Kim S, Lee JS, Lee PW. Hemorrhagic fever with renal syndrome caused by the Seoul virus. Nephron. 1995; 71(4):419–27. [Pubmed]
- Krüger DH, Ulrich R, Lundkvist A A. Hantavirus infections and their prevention. Microbes Infect. 2001 Nov; 3(13):1129–44.
[Pubmed] | [Crossref]
- Kukkonen SKJ, Vaheri A, Plyusnin A. L protein, the RNA-dependent RNA polymerase of hantaviruses. Arch Virol. 2005 Mar; 150(3):533–56. [Pubmed] | [Crossref]
- Lednicky JA. Hantaviruses. a short review. Arch Pathol Lab Med. 2003 Jan; 127(1):30–5. [Pubmed] | [Crossref]
- Lee HW, Cho HJ. Electron microscope appearance of Hantaan virus, the causative agent of Korean haemorrhagic fever. Lancet. 1981 May 16; 1(8229):1070–2. [Pubmed] | [Crossref]
- Lee HW, Johnson KM. Laboratory-acquired infections with Hantaan virus, the etiologic agent of Korean hemorrhagic fever. J Infect Dis. 1982 Nov; 146(5):645–51. [Pubmed] | [Crossref]
- Liu Y-X, Feng D, Zhang Q, Jia N, Zhao Z-T, De Vlas SJ, et al. Key differentiating features between scrub typhus and hemorrhagic fever with renal syndrome in northern China. Am J Trop Med Hyg. 2007 May; 76(5):801–5. [Pubmed] | [Source]
- Lloyd G, Bowen ET, Jones N, Pendry A. HFRS outbreak associated with laboratory rats in UK. Lancet. 1984 May 26; 1(8387):1175–6.[Pubmed] | [Crossref]
- Löber C, Anheier B, Lindow S, Klenk HD, Feldmann H. The Hantaan virus glycoprotein precursor is cleaved at the conserved pentapeptide WAASA. Virology. 2001 Oct 25; 289(2):224–9. [Pubmed] | [Crossref]
- Lundkvist A, Björsten S, Niklasson B, Ahlborg N. Mapping of B-cell determinants in the nucleocapsid protein of Puumala virus: definition of epitopes specific for acute immunoglobulin G recognition in humans. Clin Diagn Lab Immunol. 1995 Jan; 2(1):82–6. [Pubmed]
- Lundkvist A, Hukic M, Hörling J, Gilljam M, Nichol S, Niklasson B. Puumala and Dobrava viruses cause hemorrhagic fever with renal syndrome in Bosnia-Herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera. J Med Virol. 1997 Sep; 53(1):51–9. [Pubmed] | [Crossref]
- Lundkvist Å, Niklasson B. Haemorrhagic fever with renal syndrome and other hantavirus infections. Rev Med Virol. 1994 Sep 1; 4(3):177–84. [Crossref]
- Markotić A, Kuzman I, Babić K, Gagro A, Nichol S, Ksiazek TG, et al. Double trouble: hemorrhagic fever with renal syndrome and leptospirosis. Scand J Infect Dis. 2002; 34(3):221–4. [Pubmed]
- McCaughey C, Hart CA. Hantaviruses. J Med Microbiol. 2000 Jul; 49(7):587–99. [Pubmed] | [Crossref]
- Mehta S, Jiandani P. Ocular features of hantavirus infection. Indian J Ophthalmol. 2007; 55(5):378–80. [Pubmed]
- Mertz G J 2002. Bunyaviridae: Bunyaviruses, Phleboviruses, Nairoviruses and Hantaviruses; in Clinicalvirology 2n edition (eds) D D Richman, R J Whitley and F G Hayden(Washington, DC: ASM Press) pp 921–948
- Mertz GJ, Hjelle B, Crowley M, Iwamoto G, Tomicic V, Vial PA. Diagnosis and treatment of new world hantavirus infections. Curr Opin Infect Dis. 2006 Oct; 19(5):437–42. [Pubmed] | [Crossref]
- Meyer BJ, Schmaljohn CS. Persistent hantavirus infections: characteristics and mechanisms. Trends Microbiol. 2000 Feb; 8(2):61–7. [Pubmed] | [Crossref]
- Montgomery JM, Ksiazek TG, Khan AS. Hantavirus pulmonary syndrome: the sound of a mouse roaring. J Infect Dis. 2007 Jun 1; 195(11):1553–5. [Pubmed] | [Crossref]
- Morse SS, Schluederberg A. From the National Institute of Allergy and Infectious Diseases, the Fogarty International Center of the National Institutes of Health, and the Rockefeller University. Emerging viruses: the evolution of viruses and viral diseases. J Infect Dis. 1990 Jul; 162(1):1–7. [Pubmed]
- Muranyi W, Bahr U, Zeier M, Woude FJ van der. Hantavirus Infection. JASN. 2005 Dec 1; 16(12):3669–79. [Crossref]
- Nakamura T, Yanagihara R, Gibbs CJ, Gajdusek DC. Immune spleen cell-mediated protection against fatal Hantaan virus infection in infant mice. J Infect Dis. 1985 Apr; 151(4):691–7. [Pubmed] | [Crossref]
- Nichol, S. T. 2001. Bunyaviruses, p. 1603-1633. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 4th ed. Lippincott/The Williams & Wilkins Co., Philadelphia, Pa
- Okumura M, Yoshimatsu K, Kumperasart S, Nakamura I, Ogino M, Taruishi M, et al. Development of serological assays for Thottapalayam virus, an insectivore-borne Hantavirus. Clin Vaccine Immunol. 2007 Feb; 14(2):173–81. [Pubmed] | [Crossref]
- Overturf G D 2005 Clinical Sin Nombre hantaviral infections in children; Pediatr. Infect. Dis. J. 24 373–374. [Source]
- Papa A, Johnson AM, Stockton PC, Bowen MD, Spiropoulou CF, Alexiou-Daniel S, et al. Retrospective serological and genetic study of the distribution of hantaviruses in Greece. J Med Virol. 1998 Aug; 55(4):321–7. [Pubmed] | [Crossref]
- Parrington MA, Kang CY. Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus: comparison with the prototype Hantavirus. Virology. 1990 Mar; 175(1):167–75. [Pubmed]
- Peters CJ, Khan AS. Hantavirus pulmonary syndrome: the new American hemorrhagic fever. Clin Infect Dis. 2002 May 1; 34(9):1224–31. [Pubmed] | [Crossref]
- Peters CJ, Simpson GL, Levy H. Spectrum of hantavirus infection: hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. Annu Rev Med. 1999; 50:531–45. [Pubmed] | [Crossref]
- Plyusnin A. Genetics of hantaviruses: implications to taxonomy. Arch Virol. 2002 Apr; 147(4):665–82. [Pubmed]
- Plyusnin A, Vapalahti O, Vaheri A. Hantaviruses: genome structure, expression and evolution. J Gen Virol. 1996 Nov; 77 (Pt 11):2677–87. [Pubmed] | [Crossref]
- Ravkov EV, Nichol ST, Peters CJ, Compans RW. Role of actin microfilaments in Black Creek Canal virus morphogenesis. J Virol. 1998 Apr; 72(4):2865–70. [Pubmed] | [Source]
- Schmaljohn CS, Dalrymple JM. Analysis of Hantaan virus RNA: evidence for a new genus of bunyaviridae. Virology. 1983 Dec; 131(2):482–91. [Pubmed]
- Schmaljohn CS, Jennings GB, Hay J, Dalrymple JM. Coding strategy of the S genome segment of Hantaan virus. Virology. 1986 Dec; 155(2):633–43. [Pubmed]
- Schmaljohn CS, Schmaljohn AL, Dalrymple JM. Hantaan virus M RNA: coding strategy, nucleotide sequence, and gene order. Virology. 1987 Mar; 157(1):31–9. [Pubmed]
- Schmaljohn CS, Arikawa J, Hasty SE, Rasmussen L, Lee HW, Lee PW, et al. Conservation of antigenic properties and sequences encoding the envelope proteins of prototype Hantaan virus and two virus isolates from Korean haemorrhagic fever patients. J Gen Virol. 1988 Aug; 69 ( Pt 8):1949–55.[Pubmed] | [Crossref]
- Schmaljohn CS. Nucleotide sequence of the L genome segment of Hantaan virus. Nucleic Acids Res. 1990 Nov 25; 18(22):6728. [Pubmed]
- Schmaljohn CS, Chu YK, Schmaljohn AL, Dalrymple JM. Antigenic subunits of Hantaan virus expressed by baculovirus and vaccinia virus recombinants. J Virol. 1990 Jul; 64(7):3162–70. [Pubmed] | [Source]
- Schmaljohn C, Hjelle B. Hantaviruses: a global disease problem. Emerging Infect Dis. 1997 Jun; 3(2):95–104. [Pubmed] | [Crossref]
- Severson W, Xu X, Kuhn M, Senutovitch N, Thokala M, Ferron F, et al. Essential amino acids of the hantaan virus N protein in its interaction with RNA. J Virol. 2005 Aug; 79(15):10032–9. [Pubmed] | [Crossref]
- Sjölander KB, Elgh F, Kallio-Kokko H, Vapalahti O, Hägglund M, Palmcrantz V, et al. Evaluation of serological methods for diagnosis of Puumala hantavirus infection (nephropathia epidemica). J Clin Microbiol. 1997 Dec; 35(12):3264–8. [Pubmed] | [Source]
- Sjölander KB, Lundkvist A. Dobrava virus infection: serological diagnosis and cross-reactions to other hantaviruses. J Virol Methods. 1999 Jul; 80(2):137–43. [Pubmed] | [Crossref]
- Spiropoulou CF, Morzunov S, Feldmann H, Sanchez A, Peters CJ, Nichol ST. Genome structure and variability of a virus causing hantavirus pulmonary syndrome. Virology. 1994 May 1; 200(2):715–23. [Pubmed] | [Crossref]
- Stohwasser R, Giebel LB, Zöller L, Bautz EK, Darai G. Molecular characterization of the RNA S segment of nephropathia epidemica virus strain Hällnäs B1. Virology. 1990 Jan; 174(1):79–86. [Pubmed]
- Frey MT, Vial PC, Castillo CH, Godoy PM, Hjelle B, Ferrés MG. Hantavirus Prevalence in the IX Region of Chile. Emerg Infect Dis. 2003 Jul; 9(7):827–32. [Pubmed] | [Crossref]
- Ulrich R, Hjelle B, Pitra C, Krüger DH. Emerging viruses: the case “hantavirus.” Intervirology. 2002; 45(4-6):318–27. [Pubmed]
- Van Regenmortel M H V, Fauquet C M, Bishop D H L, Carstens E B , Estes M K, Lemon S M, Maniloff J, Mayo M A, McGeoch D J, Pringle C R and Wickner R B 2000 Virus Taxonomy Seventh Report of the International Committee on Taxonomy of Viruses (San Diego: Academic Press)
- Van Ypersele de Strihou C. Clinical features of hemorrhagic fever with renal syndrome in Europe. Kidney Int Suppl. 1991 Dec; 35:S80–3. [Pubmed]
- Vapalahti O, Kallio-Kokko H, Närvänen A, Julkunen I, Lundkvist A, Plyusnin A, et al. Human B-cell epitopes of Puumala virus nucleocapsid protein, the major antigen in early serological response. J Med Virol. 1995 Aug; 46(4):293–303. [Pubmed]
- Vapalahti O, Lundkvist A, Vaheri A. Human immune response, host genetics, and severity of disease. Curr Top Microbiol Immunol. 2001; 256: 153–69. [Pubmed]
- Wells RM, Sosa Estani S, Yadon ZE, Enria D, Padula P, Pini N, et al. An unusual hantavirus outbreak in southern Argentina: person-to-person transmission? Hantavirus Pulmonary Syndrome Study Group for Patagonia. Emerg Infect Dis. 1997; 3(2):171–4. [Pubmed]
- Wichmann D, Slenczka W, Alter P, Boehm S, Feldmann H. Hemorrhagic Fever with Renal Syndrome: Diagnostic Problems with a Known Disease. J Clin Microbiol. 2001 Sep; 39(9):3414–6. [Pubmed] | [Crossref]
- Xiao SY, Leduc JW, Chu YK, Schmaljohn CS. Phylogenetic analyses of virus isolates in the genus Hantavirus, family Bunyaviridae. Virology. 1994 Jan; 198(1):205–17. [Pubmed] | [Crossref]
- Yadav PD, Vincent MJ, Nichol ST. Thottapalayam virus is genetically distant to the rodent-borne hantaviruses, consistent with its isolation from the Asian house shrew (Suncus murinus). Virol J. 2007 Aug 21; 4:80. [Pubmed] | [Crossref]
- Young JC, Mills JN, Enria DA, Dolan NE, Khan AS, Ksiazek TG. New World hantaviruses. Br Med Bull. 1998; 54(3):659–73. [Pubmed] | [Source]
- Zeller HG, Karabatsos N, Calisher CH, Digoutte JP, Cropp CB, Murphy FA, et al. Electron microscopic and antigenic studies of uncharacterized viruses. II. Evidence suggesting the placement of viruses in the family Bunyaviridae. Arch Virol. 1989; 108(3-4):211–27. [Pubmed]
- Zöller L, Yang S, Gött P, Bautz EK, Darai G. Use of recombinant nucleocapsid proteins of the Hantaan and nephropathia epidemica serotypes of Hantaviruses as immunodiagnostic antigens. J Med Virol. 1993 Mar; 39(3):200–7.